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KMID : 0381920070370040209
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Korean Journal of Microscopy
2007 Volume.37 No. 4 p.209 ~ p.217
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The Temporal and Spatial Expression of the Cytokeratin in Keratinocytes during Cutaneous Wound Healing on the Amphibian (Bombina orientalis)
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Lim Do-Seon
Jeong Soon-Jeong
Moon Myung-Jin
Jeong Moon-Jin
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Abstract
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The system of wound healing is very complex biological processing that includes inflammatory, reepithelialization, and matrix construction. For identification of the transitional pathway of the keratinocytes, we have employed immunohistochemical analysis using cytokeratin antibody after wounding. Epithelium in skin of the frog (Bombina orientalis) was examined with transmission electron microscopy. Cytokeratin was expressed in normal basal and gland cavity cells. At 3-hour basal layer cells were strong positive, however cells of the upper layer were negative reaction. Day1 and 2 after post-wounding, regenerating epithelial cell layer was positive reaction, especially basal layer cells were strong positive. At day 10 after wounding, the degree of positive reaction to basal cells of regenerating epithelial tissue was equal to day 7 wound tissue. At day of 19th, basal and spinous layer cells were strong positive reaction. Regenerating epithelial cells were positive but some basal cells were strong positive at day 27. From this result, we identified that the migration of the keratinocytes in amphibian skin wounds is initiated from basal layer cells and the keratinocytes migrate into basal and middle of the wound area.
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KEYWORD
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Amphibian, Bombina orientalis, Cutaneous, Cytokeratin, Keratinocyte, Wound healing
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